Facets

1979

#6901

Resolution of the ATP-dependent proteolytic system from reticulocytes: a component that interacts with ATP.

Discovery of ubiquitin-mediated protein degradation. The paper is available at doi:10.1073/pnas.76.7.3107,PMC 383772, PMID 290989. See also Hershko, A.; Ciechanover, A.; Heller, H.; Haas, A.L.; Rose, I.A. (1980), "Pro…

1981

#14183

Computer averaging of electron micrographs of the 405 ribosomal subunit.

Frank and colleagues developed a method that allows sorting of particle images into classes based on their orientation, as well as their structural features. Specifically Frank developed mathematical tools used for im…

1981

#14224

The crystallization of ribsomal proteins from the 50 S subunit of the Escherichia coli and Bacillus stearothermophilus ribosome.

The authors crystallized fragments of the 50S subunit of a thermophile bacterium’s ribosome to 3 angstroms resolution. Order of authorship in the original publication: Appelt, Dyck, et al., Yonath. Digital facsimile f…

1982

#13932

Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena.

Discovery of ribozymes (ribonucleic acid enzymes). Cech discovered that RNA itself could cut strands of RNA, suggesting that life might have started as RNA. "In the 1970s, Cech had been studying the splicing of RNA in…

1983

#14140

Mammalian beta-adrenergic receptors. Structural differences in beta 1 and beta 2 subtypes revealed by peptide maps.

Lefkowitz and colleagues showed that there are two different types of beta receptors, distinguishing them as Beta-1 and Beta-2. They noticed that each has specific pharmacological characteristics. Order of authorship …

1985

#10785

Enzymatic amplication of B-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Polymerase chain reaction first published. With Randall K. Saiki, Stephen Scharf, Fred Faloona et al. Order of authorship in the original paper was Saiki, Scharf, Faloona, Mullis.... In 1993 the Nobel Prize in Chemist…

1985

#11060

Structure of the protein subunit in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3 Å resolution.

Discovery of the three-dimensional structure of a protein complex found in certain photosynthetic bacteria, called the photosynthetic reaction center. This was the first elucidation of the 3D crystal structure of any …

1985

#14339

Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface.

In this paper Smith invented "phage display technology," a technique where a specific protein sequence is artifically inserted into the coat protein gene of a bacteriophage, causing the protein to be expressed on the …

1990

#14340

Phage antibodies: Filamentous phage displaying antibody variable domains.

Working in the Laboratory of Molecular Biology at Cambridge University, Winter became interested in the idea that all antibodies have the same basic structure, with only small changes making them specific for one targ…

1993

#14341

Tuning the activity of an enzyme for unusual environments: Sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide.

Arnold introduced a biochemical molecule manipulating technique to mimic the process of natural selection in creating new enzymes adapted to a specific catalytic reaction. She directed evolution of subtilisin E to obt…

1994

#13564

Green fluorescent protein as a marker for gene expression.

Chalfie and colleagues showed that the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, could be used as a visible marker for protein localization and expression in vivo, in bacteria and worm cell…

1994

#14314

Structure at 2.8 Â resolution of F1-ATPase from bovine heart mitochondria.

Walker used X-ray crystallography to determine the structure of ATP synthase (ATPase or adenosine triphosphatase). In 1997 Walker shared the Nobel Prize in Chemistry with Paul Boyer and Jens C. Skou “for their elucida…

1996

#13565

Crystal structure of Aequorea victoria green fluorescent protein.

Tsien and colleagues published the crystal structure of the 238 A.A. long green fluorescent protein (GFP). With this data, the authors could determine what had to be modified within the protein in such a way that the …

1998

#14332

The structure of the potassium channel: Molecular basis of K+ conduction and selectivity.

The authors determined the first high resolution structure of an ion channel, called KcsA from the bacterium Streptomyces lividans. The structure that they revealed was perfectly adapted to allow entry of potassium io…

2000

#13952

Structure of the 30S ribosomal subunit.

Ramakrishnan and colleagues determined the complete molecular structure of the 30S subunit of the ribosome and its complexes with several antibiotics. " The Abstract: "Genetic information encoded in messenger RNA is t…

2000

#13953

The structural basis of ribosome activity in peptide bond synthesis.

With Poul Nissen, Jeffrey Hansen, Nenad Ban, Peter B. Moore. Steitz shared the 2009 Nobel Prize in Chemistry with Venkatraman Ramakrishnan and Ada Yonath "for studies of the structure and function of the ribosome."

2000

#13982

Architecture of RNA polymerase II and implications for the transcription mechanism.

Kornberg devoted two decades to the development of methods to visualize the atomic structure of RNA polymerase and its associated protein components. Initially, Kornberg took advantage of expertise with lipid membrane…

2000

#14293

Cell surface engineering by a modified Staudinger reaction.

In 2022 Carolyn Bertozzi shared the Nobel Prize in Chemistry with Barry Sharpless and Morten Meldal for discovery of click chemistry. Bertozzi invented a biorthogonal variation of Sharpless and Meldal reactions. In th…

2001

#14291

Click chemistry: Diverse chemical function from a few good reactions.

In 2022 Barry Sharpless shared the Nobel Prize in Chemistry with Carolyn Bertozzi and Morten Meldal for "the discovery of click chemistry." "Barry Sharpless coined the concept of click chemistry, where molecular build…

2002

#14292

Peptidotriazoles on Solid Phase: [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides.

In 2022 Morten Meldal shared the Nobel Prize in Chemistry with Carolyn Bertozzi and Barry Sharpless for the discovery of click chemistry. Simultaneously, but independently of Sharpless, the authors discovered that cop…

2002

#14333

Crystal structure and mechanism of a calcium gated potassium channel.

The authors first obtained the crystal structure of a Ca2+ - gated K+ ion channel and then deciphered how ion channels open and close, a process called "gating" in response to cues in their environment. They determine…

2003

#14300

Design of a novel globular protein fold with atomic-level accuracy.

Called, "the breakthrough in computational de novo protein design." This was the proof of concept paper that computers and AI could be used to predict protein structures accurately and much faster than with convention…

2006

#13094

The Pauling catalogue. Ava Helen and Linus Pauling papers at Oregon State University. Edited by Christoffer Petersen and Cliff Mead. 6 vols.

Vol. 1. Timeline, correspondence, publications, manuscripts & typescripts of articles, speechs, and books. Vol. 2. Science, Research Notebooks. 1917 Linus Pauling Diary. Vol. 3. Peace, Ava Helen Pauling, travel, honor…

2011

#14134

Crystal structure of the β2 adrenergic receptor–Gs protein complex.

Kobilka and colleagues published the crystal structure of a beta-2 receptor forming a complex with the G protein coupled receptor. This was the first time that a complete complex of an active receptor and it's Gs prot…

2012

#11844

A programmable dual RNA-guided DNA endonuclease in adaptive bacterial immunity.

Order of authorship in the original publication: Jinek, Chylinski, Fonfar, Hauer, Doudna, Charpentier. Doudna, Charpentier and colleagues showed for the first time that the CRISPR evolutionary immune tool of bacteria …

2021

#14026

Highly accurate protein structure prediction with AlphaFold.

Abstract: "Proteins are essential to life, and understanding their structure can facilitate a mechanistic understanding of their function. Through an enormous experimental effort1,2,3,4, the structures of around 100,0…