Expression in Escherichia coli of chemically synthesized genes for human insulin (plasmid construction / lac operon / fused proteins / radioimmunoassay / peptide purification).

Working at Genentech, Kleid and Goeddel and colleagues were the first scientists to apply genetic engineering techniques, incorporating chemically synthesized DNA encoding human insulin into E. coli, that expressed, after appropriate treatment, synethetic human insulin. Digital facsimile from PubMedCentral at this link.