Saggio di osservazioni microscopiche concernenti il sistema della generazione dei Signori de Needham e Buffon. IN: Dissertazione due… pp. [2]-87.

Spallanzani, a believer in preformation theory, found that he could prevent contamination by microorganisms in strongly heated infusions protected from aerial contamination, but he observed that as soon as air was allowed to enter the flask, microorganisms proliferated. He was one of the first to dispute the doctrine of spontaneous generation. Digital facsimile from Google Books at this link.