Demostracion de las eficaces virtudes nuevamente descubiertas en las raices de dos plantas de Nueva-España, especies de ágave y de begónia, para la curacion del vicio venéreo y escrofuloso ...

Balmis conducted experimental trials on the effectiveness of two Mexican plants, agave and begonia, which were believed, according to folk medicine practices in Mexico, to cure syphilis and scrofula. The trials confirmed that the plants were ineffective. He published the results in a deluxe book with two handsome hand-colored plates.